Customers often ask "How do I determine the best annealing temperature (Ta) for my primers?"
To determine the best annealing temperature for the primers using the site-directed mutagenesis kit, we recommend that you use the NEBbase changer tool. The NEBbase changer tool takes into account the mismatches in your mutagenic primer set. Standard Tm calculators do not take into account those mismatches, so the Tm that you get from those tools may not be optimum.
Remember, if you have any other questions you can always reach us at info@neb.com.
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
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