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Product Information
Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications. Highlights
- DH5α™ derivative
- Free of animal products
- Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR)
- Tight control of expression by laclq allows potentially toxic genes to be cloned
- Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
- Resistance to phage T1 (fhuA2)
- Suitable for blue/white screening by α-complementation of the β-galactosidase gene
- F' allows cells to be infected with bacteriophage M13 for ssDNA production
- Reduced recombination of cloned DNA (recA1)
- K12 Strain
Transformation Efficiency
1-3 x 109 cfu/µg pUC19 DNAGenotype
F´ proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (TetR) / fhuA2∆(argF-lacZ)U169 phoA glnV44 Φ80Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17- This product is related to the following categories:
- Cloning Competent Cell Strains Products
- This product can be used in the following applications:
- Transformation,
- High-throughput cloning and automation solutions
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Protocols, Manuals & Usage
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Tools & Resources
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FAQs & Troubleshooting
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Citations & Technical Literature
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Product Citation Tool
Additional Citations
- Firnberg E, Ostermeier M (2013) The genetic code constrains yet facilitates Darwinian evolution Nucleic Acids Res; 41(15), 7420-8. PubMedID: 23754851, DOI: 10.1093/nar/gkt536
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