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BSA, Molecular Biology Grade

BSA, Molecular Biology Gradeカタログ番号:B9000

カタログ番号

サイズ

濃度

価格

保存温度

B9000S 12 mg 20 mg/ml 在庫限りで販売終了、代替品:B9200S -20C

製品カテゴリ>グループ

  • 制限酵素>その他
  • その他(バッファー、研究用備品)>制限酵素バッファー

備考

  • 備考:販売終了(2022年12月)代替品:B9200S

特徴

説明:

Bovine Serum Albumin (BSA)は反応チューブやピペット表面への酵素の吸着を防ぐために使用する。またインキュベーション中のタンパク質の安定化作用も有している。

 

販売終了製品(BSA, #9001S)との違い:

(1)品質

製造方法の変更と品質管理の追加(以下品質管理を参照)により、精製度がさらに向上

 

(2)濃度

・BSA (B9001S): 10 mg/ml

・BSA, Molecular Biology Grade (B9000S): 20 mg/ml

 

(3)保存溶液

・BSA (B9001S): 20 mM KPO4 (pH 7.0), 50 mM NaCl, 0.1 mM EDTA and 5% Glycerol

・BSA, Molecular Biology Grade (B9000S): 20 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 50% Glycerol

 

(4)形状

・BSA (B9001S): 4本

・BSA, Molecular Biology Grade (B9000S): 1本(600 ul)

 

保存温度:

-20℃

 

保存溶液:

・20 mM Tris-HCl
・100 mM KCl
・0.1 mM EDTA
・50% Glycerol
pH 8.0 @ 25°C

品質管理

以下品質管理試験に基づいて各ロットが検証されている。

 

Endonuclease Activity (Nicking):
The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.

 

Exonuclease Activity (Radioactivity Release):
The product is tested in a reaction containing a radiolabeled mixture of single and double-stranded DNA. After incubation for 4 hours the exonuclease activity is determined by the % release of radioactive nucleotides.

 

Non-Specific DNase Activity (16 hour):
The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.

 

Phosphatase activity (FAM labeled oligo):
The product is tested in a reaction containing a fluorescent internal labeled single stranded oligonucleotide with a 5' phosphate. The percent removal of the 5' phosphate is determined by capillary electrophoresis.

 

Protein Concentration (A280):
The protein concentration is determined by A280 spectroscopy using the Pace method (Pace, C.N. et al. (1995) Protein Sci., 4, 2411–2423).

 

qPCR DNA Contamination (E. Coli Genomic):
The product is screened for the presence of E. coli genomic DNA using SYBR® Green qPCR with primers specific for the E.coli 16S rRNA locus. Results are quantified using a standard curve generated from purified E. coli genomic DNA.  The measured level of E. coli genomic DNA contamination is less than 1 E.coli genome.

 

RNase Activity (2 Hour Digestion):
The product is tested in a reaction containing a RNA substrate.  After incubation for 2 hours there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.

 


RNase Activity (Extended Digestion):
The product is tested in a reaction containing a RNA substrate. After incubation for 16 hours greater than 90% of the substrate RNA remains intact as determined by gel electrophoresis.


Single Stranded DNase Activity (FAM Labeled Oligo):
The product is tested in a reaction containing a fluorescent internal labeled single stranded oligonucleotide. The percent degradation is determined by capillary electrophoresis.

参考文献

  1. Pace, C.N. et al. (1995). Protein Sci,. 4, 2411-2423.