BSA, Molecular Biology Grade
カタログ番号 |
サイズ |
濃度 |
価格 |
保存温度 |
|---|---|---|---|---|
| B9000S | 12 mg | 20 mg/ml | 販売終了、代替品：B9200S | -20C |
製品カテゴリ>グループ
- 制限酵素>その他
- その他(バッファー、研究用備品)>制限酵素バッファー
特徴
説明：
Bovine Serum Albumin (BSA)は反応チューブやピペット表面への酵素の吸着を防ぐために使用する。またインキュベーション中のタンパク質の安定化作用も有している。
販売終了製品(BSA, #9001S)との違い：
（１）品質
製造方法の変更と品質管理の追加（以下品質管理を参照）により、精製度がさらに向上
（２）濃度
・BSA (B9001S): 10 mg/ml
・BSA, Molecular Biology Grade (B9000S)： 20 mg/ml
（３）保存溶液
・BSA (B9001S): 20 mM KPO4 (pH 7.0), 50 mM NaCl, 0.1 mM EDTA and 5% Glycerol
・BSA, Molecular Biology Grade (B9000S)： 20 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 50% Glycerol
（４）形状
・BSA (B9001S): 4本
・BSA, Molecular Biology Grade (B9000S)： 1本（600 ul)
保存温度：
-20℃
保存溶液：
・20 mM Tris-HCl
・100 mM KCl
・0.1 mM EDTA
・50% Glycerol
pH 8.0 @ 25°C
プロトコール
品質管理
以下品質管理試験に基づいて各ロットが検証されている。
Endonuclease Activity (Nicking):
The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
Exonuclease Activity (Radioactivity Release):
The product is tested in a reaction containing a radiolabeled mixture of single and double-stranded DNA. After incubation for 4 hours the exonuclease activity is determined by the % release of radioactive nucleotides.
Non-Specific DNase Activity (16 hour):
The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
Phosphatase activity (FAM labeled oligo):
The product is tested in a reaction containing a fluorescent internal labeled single stranded oligonucleotide with a 5' phosphate. The percent removal of the 5' phosphate is determined by capillary electrophoresis.
Protein Concentration (A280):
The protein concentration is determined by A280 spectroscopy using the Pace method (Pace, C.N. et al. (1995) Protein Sci., 4, 2411–2423).
qPCR DNA Contamination (E. Coli Genomic):
The product is screened for the presence of E. coli genomic DNA using SYBR® Green qPCR with primers specific for the E.coli 16S rRNA locus. Results are quantified using a standard curve generated from purified E. coli genomic DNA. The measured level of E. coli genomic DNA contamination is less than 1 E.coli genome.
RNase Activity (2 Hour Digestion):
The product is tested in a reaction containing a RNA substrate. After incubation for 2 hours there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.
RNase Activity (Extended Digestion):
The product is tested in a reaction containing a RNA substrate. After incubation for 16 hours greater than 90% of the substrate RNA remains intact as determined by gel electrophoresis.
Single Stranded DNase Activity (FAM Labeled Oligo):
The product is tested in a reaction containing a fluorescent internal labeled single stranded oligonucleotide. The percent degradation is determined by capillary electrophoresis.
参考文献
- Pace, C.N. et al. (1995). Protein Sci,. 4, 2411-2423.
