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Product Information
Chemically competent E. coli cells suitable for high efficiency transformation and isolation of plasmid clones containing repeat elements.
Highlights
- Recommended host strain for cloning genes into retroviral/ lentiviral vectors
- Recommended for cloning of direct repeats and inverted repeat sequences
- Reduced recombination of cloned DNA (recA1)
- Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]
- Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
- Resistance to phage T1 (fhuA)
- Suitable for blue/white screening with vectors capable of alpha-complementation
Transformation Efficiency
1-3 x 109 cfu/µg pUC19 DNAGenotype
F' proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (TetR)/∆(ara-leu) 7697 araD139 fhuA ∆lacX74 galK16 galE15 e14- Φ80dlacZ∆M15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 ∆(mrr-hsdRMS-mcrBC)- This product is related to the following categories:
- Cloning Competent Cell Strains Products
- This product can be used in the following applications:
- Transformation,
- High-throughput cloning and automation solutions
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Protocols, Manuals & Usage
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Traditional Cloning Workflow
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NEBUILDER HIFI DNA ASSEMBLY® Removal of 3´ end Mismatches
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Fast transformation plating with glass beads
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