α2-3,6,8,9 Neuraminidase A

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
P0722S     -20       α2-3,6,8,9 Neuraminidase A P0722SVIAL -20 1 x 0.04 ml 20,000 units/ml   GlycoBuffer 1 B1727SVIAL -20 1 x 1 ml 10 X
P0722L     -20       α2-3,6,8,9 Neuraminidase A P0722LVIAL -20 1 x 0.2 ml 20,000 units/ml   GlycoBuffer 1 B1727SVIAL -20 1 x 1 ml 10 X

Companion Products

• PNGase F, Recombinant
• PNGase F (Glycerol-free), Recombinant
• PNGase F
• PNGase F (Glycerol-free)
• O-Glycosidase
• α2-3,6,8 Neuraminidase
• α2-3 Neuraminidase S

1. Reactions may be scaled-up linearly to accommodate larger reaction volumes.
2. The amount of exoglycosidase enzyme required varies when different substrates are used. Start with 1–2 μl for 1 μg of glycoprotein or 100 nM of oligosaccharide for one hour in a 10–25 μl reaction. If there is still undigested material, let the reaction go overnight.
3. Higher concentrations of enzyme as well as longer incubation times may be necessary for cleavage of branched structures.

1. Iwamori, M., Ohta, Y., Uchida, Y. and Tsukada, Y. (1997). Glycocon. J. 1, 67-73.
2. Wong-Madden, S. T. and Landry, D. (1995). Glycobiology. 5, 19-28.

1. Typical Reaction Conditions for α2-3,6,8,9 Neuraminidase A (P0722)

• Detailed Characterization of Antibody Glycan Structure using the N-Glycan Sequencing Kit

• Glycan Analyzer

1. What is the difference between the four Neuraminidase enzymes sold by NEB: α2-3,6,8 Neuraminidase, α2-3,6,8,9 Neuraminidase A, α2-3 Neuraminidase and α2-3 Neuraminidase S?
2. What is a good positive control for α2-3,6,8,9 Neuraminidase A?
3. Can I use α2-3,6,8,9 Neuraminidase A in a double digest with other exoglycosidases and/or endoglycosidases?
4. Can I use α2-3,6,8,9 Neuraminidase A in a double digest with Endo H/Hf, O-Glycosidase or PNGase F?
5. Does this enzyme require denaturing conditions to act on glycoproteins?
6. What is a good method to re-purify a glycan or glycopeptide after exoglycosidase treatment?
7. Do detergents inhibit glycosidases?
8. What are Glycosidases and their uses?
9. Do the NEB Neuraminidase enzymes cleave both N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) residues?
10. Is it necessary to treat my glycoprotein concomitantly with Neuraminidase and O-Glycosidase?
11. How much exoglycosidase should be used?
12. How much exoglycosidase should be used?

• Glycoproteomics Brochure
• Glycoproteomics Technical Guide

• Albrecht, S., Vainauskas, S., Stöckmann, H., McManus, C., Taron, C.H. and Rudd, P.M. (2016) Comprehensive Profiling of Glycosphingolipid Glycans Using a Novel Broad Specificity Endoglycoceramidase in a High-Throughput Workflow. Anal Chem; May 3;88(9), 4795-802. Anal Chem.. PubMedID: 27033327

• Using Glycosidases to Remove Trim or Modify Glycans on Therapeutic Protein
• Redesigning glycosidase manufacturing quality for pharmaceutical and clinical applications

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• P0722S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• P0722S_L_v1_0021602
• P0722S_L_v1_0011605
• P0722S_L_v1_0021608
• P0722S_L_v1_0021612
• P0722S_L_v1_0021706
• P0722S_L_v1_0021710
• P0722S_L_v1_0021801
• P0722S_L_v1_0021803
• P0722L_v1_10020203
• P0722S_v1_10020388
• P0722S_v1_10022690
• P0722L_v1_10028396
• P0722S_v1_10031676
• P0722L_v1_10040007
• P0722S_v1_10040005
• P0722S_v1_10057252
• P0722L_v1_10064911
• P0722S_v1_10064910
• P0722L_v1_10070591
• P0722S_v1_10075161
• P0722S_v1_10079100
• P0722L_v1_10084288
• P0722S_v1_10084289
• P0722L_v1_10091048
• P0722S_v1_10110147
• P0722S_v1_10110741
• P0722L_v1_10110150
• P0722S_v1_10118372
• P0722L_v1_10124251
• P0722L_v1_10057251
• P0722L_v1_10138069
• P0722S_v1_10130273
• P0722S_v1_10138071
• P0722L_v1_10155099
• P0722S_v1_10178410
• P0722L_v1_10187148
• P0722S_v1_10197174
• P0722L_v1_10219197
• P0722S_v1_10228341

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• α2-3,6,8,9 Neuraminidase A
• GlycoBuffer 1

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.