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Monarch RNA Cleanup Kit (50 μg)

Monarch RNA Cleanup Kit (50 μg) カタログ番号:T2040

カタログ番号

サイズ

濃度

価格

保存温度

T2040S 10 preps ¥9,100 室温
T2040L 100 preps ¥51,000 室温

製品カテゴリ>グループ

  • 核酸精製キット>RNAクリーンアップ

特徴

in vitro 転写反応などのクリーンアップに最適な RNA 精製キット (50 µg)

 

特長:

 ・in vitro 転写反応などの RNA 酵素反応中の RNA クリーンアップが可能

 ・DNase I や Proteinase K 処理した Total RNA の精製も可能

 ・RT-qPCR や NGS ライブラリー調製のための RNA クリーンアップに最適

 ・RNA サイズ : ≧ 25 nt (オプション・プロトコールにより ≧15 ntも可能)
 ・溶出液量 : ≧ 20 ul
 ・一般的な回収率 : 70 - 100 %
 ・純度 : A260/280 > 1.8、A260/230 > 1.8

 

  モナーク RNA クリーンアップ・プロトコール

image

 

 

The Monarch RNA Cleanup Kit enables efficient recovery of RNA in as little as 20 µl

image

  rRNA (50 µg of 16S and 23S Ribosomal Standard from E. coli, Sigma) was purified using the Monarch RNA Cleanup Kit (50 µg, NEB #2040) and eluted with nuclease-free water using the elution volumes indicated. The percent recovery of RNA was calculated from the resulting A260, as measured using a Trinean DropSense™ 16. Approximately 80% of RNA can be efficiently recovered in as little as 6 µl.  

 

 

The Monarch RNA Cleanup Kit (50 µg) produces sgRNA yields consistent with other competitor RNA cleanup kits and with lower residual NTP contamination
  image
 

Six different sgRNA synthesis reactions from the EnGen® sgRNA Synthesis Kit, S. pyogenes (NEB #E3322) were cleaned up using either the Monarch RNA Cleanup Kit (50 µg, NEB #T2040) or a competitor kit (according to manufacturer’s recommendations) and eluted with 50 µl of nuclease-free water. sgRNA yield was calculated from the resulting A260, as measured using a Trinean Dropsense™ 16. The Monarch Cleanup Kit produced sgRNA yields consistent with other commercially available RNA cleanup kits. 


Following cleanup, residual nucleotides (NTPs) were measured by LC-MS and are reported as (percent area NTPs (rATP+rCTP+rGTP+rUTP)/percent area sgRNA). The Monarch RNA Cleanup Kit consistently outperforms other commercially available RNA cleanup kits in the removal of residual NTPs from sgRNA synthesis reactions. 

 

 

キット内容

カラム単品とバッファー単品も販売しています。

Monarch RNA Cleanup Column (50 µg)

Monarch RNA Cleanup Binding Buffer

Monarch RNA Cleanup Wash Buffer

Nuclease-free Water

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