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- Monarch RNA Cleanup Kit (50 μg)
Monarch RNA Cleanup Kit (50 μg)
Monarch RNA Cleanup Kit (50 μg) カタログ番号:T2040
カタログ番号 |
サイズ |
濃度 |
価格 |
保存温度 |
| T2040S |
10 preps |
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¥9,100 |
室温 |
| T2040L |
100 preps |
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¥51,000 |
室温 |
特徴
| in vitro 転写反応などのクリーンアップに最適な RNA 精製キット (50 µg) |
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特長：
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| ・in vitro 転写反応などの RNA 酵素反応中の RNA クリーンアップが可能 |
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・DNase I や Proteinase K 処理した Total RNA の精製も可能
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・RT-qPCR や NGS ライブラリー調製のための RNA クリーンアップに最適
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| ・RNA サイズ : ≧ 25 nt (オプション・プロトコールにより ≧15 ntも可能) |
| ・溶出液量 : ≧ 20 ul |
| ・一般的な回収率 : 70 - 100 % |
| ・純度 : A260/280 > 1.8、A260/230 > 1.8 |
| モナーク RNA クリーンアップ・プロトコール |
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| The Monarch RNA Cleanup Kit enables efficient recovery of RNA in as little as 20 µl |
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rRNA (50 µg of 16S and 23S Ribosomal Standard from E. coli, Sigma) was purified using the Monarch RNA Cleanup Kit (50 µg, NEB #2040) and eluted with nuclease-free water using the elution volumes indicated. The percent recovery of RNA was calculated from the resulting A260, as measured using a Trinean DropSense™ 16. Approximately 80% of RNA can be efficiently recovered in as little as 6 µl. |
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| The Monarch RNA Cleanup Kit (50 µg) produces sgRNA yields consistent with other competitor RNA cleanup kits and with lower residual NTP contamination |
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Six different sgRNA synthesis reactions from the EnGen® sgRNA Synthesis Kit, S. pyogenes (NEB #E3322) were cleaned up using either the Monarch RNA Cleanup Kit (50 µg, NEB #T2040) or a competitor kit (according to manufacturer’s recommendations) and eluted with 50 µl of nuclease-free water. sgRNA yield was calculated from the resulting A260, as measured using a Trinean Dropsense™ 16. The Monarch Cleanup Kit produced sgRNA yields consistent with other commercially available RNA cleanup kits.
Following cleanup, residual nucleotides (NTPs) were measured by LC-MS and are reported as (percent area NTPs (rATP+rCTP+rGTP+rUTP)/percent area sgRNA). The Monarch RNA Cleanup Kit consistently outperforms other commercially available RNA cleanup kits in the removal of residual NTPs from sgRNA synthesis reactions.
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